Isolation and identification of phosphate solubilizing bacteria from the rhizosphere of rice in organic and non-organic rice fields in Sukoharjo District, Indonesia

Abstract. Purwanti D, Setyaningsih R, Susilowati A. 2019. Isolation and identification of phosphate solubilizing bacteria from the rhizosphere rice in organic non-organic fields Sukoharjo District, Indonesia. Bonorowo Wetlands 9: 108-115. One essential nutrients soil is phosphate. Phosphate fertilization often inefficient because bound difficult for plants to absorb. The use (PSB) can be used as an alternative availability P soil. This study aimed obtain PSB isolates with high solubilization ability identify these molecularly. Soil samples were taken (Oryza sativa L.) Sukoharjo, Central Java, Indonesia, namely Grogol Village, Menuran Pondok Village. research activity was carried out several stages: isolation on Pikovskaya medium, characterization testing dissolving P, Gram staining. colonies a index showed different colony colors taken, then DNA isolated. by extraction method using kit. gene encoding 16S rRNA amplified obtained samples. Sequencing genetic analyzer. Data analysis comparing sequence data BLASTN program. Based paddy fields, 14 obtained, ten PSB. P13, P1, Q7, Q8 had highest dissolution (IP), 3.35, 2.13, 1.82, 1.47. that P1 P13 99% similarity Acinetobacter sp. ADP1 strain. isolate Q7 96% genus Clavibacter strain NCPPB 382, ??while Pseudomonas aeruginosa PAO1.